薯蓣皂苷对动脉粥样硬化大鼠白介素-6的影响

The effect of diosgenin on interleukin-6 in atherosclerosis rats

目的 探讨薯蓣皂苷对动脉粥样硬化模型大鼠白介素6（interleukin-6，IL-6）的影响。方法 采用随机数字表法将60只雄性SD大鼠随机分成：假手术组、模型组、阿司匹林组、薯蓣皂苷低剂量组及高剂量组。饲养7 d后，除假手术组，余大鼠于左侧颈总动脉行主动脉内膜球囊拉伤术。3个月后，行颈总动脉HE染色，采用图象分析仪测定颈总动脉血管腔面积（L）、血管壁（中膜）面积（W），并计算其比值（W/L），检测血清IL-6，Western blot测定主动脉IL-6表达。结果 与模型组颈动脉损伤侧W/L （0.803±0.043）相比，薯蓣皂苷高、低剂量组颈动脉损伤侧W/L（0.615±0.040、0.753±0.043）均降低，且高剂量组小于阿司匹林组（0.759±0.049）。与模型组血清IL-6[（59.636±4.874）pg/ml]比较，薯蓣皂苷高、低剂量组[（42.582±4.724）pg/ml、（49.055±4.791）pg/ml]均降低，且高剂量组低于阿司匹林组[（52.563±4.581）pg/ml]。薯蓣皂苷高、低剂量组血管壁IL-6表达[（0.296±0.016）pg/ml、（0.435±0.015）pg/ml]低于模型组（0.455±0.026）pg/ml，高剂量组IL-6表达低于阿司匹林组（0.426±0.012）pg/ml。结论 薯蓣皂苷可降低实验性动脉粥样硬化（AS）大鼠血清及动脉壁IL-6水平。

Objective To investigate the effect of diosgenin tablet on interleukin-6 in the serum and artery of experimental atherosclerosis（AS）rats. Methods Sixty male SD rats were assigned randomly into five groups： group of sham operation（GSO, n=12）, group of model（GM, n=12）, group of Aspirin（GA, n=12）, group of Diosgenin tablet in small dosage（GDS, n=12）, and group of Diosgenin tablet in large dosage（GDL, n=12）. After 7 days, the balloon-injured carotid artery angioplasty was performed except GSO. After 3 months, rats were killed. The rats’ carotid arteries were stained with hematoxylin-eosin. Image analysis system was applied to measure the area of lumina and the thickness of wall to evaluate the ratio of W to L. Their blood IL-6 was detected. The expression of IL-6 in the aorta was measured with western blot. Results In the injured side, the W/L ratios of carotid artery in GDL and GDS（0.615±0.040、0.753±0.043）were much thinner than that of model group（0.803±0.043）. That of GDL was thinner than GA（0.759±0.049）. Compared with the model group（59.636±4.874）pg/ml, the level of serum IL-6 was decreased in GDL and GDS[（42.582±4.724）pg/ml、（49.055±4.791）pg/ml], GDL was much lower than GA[（52.563±4.581）pg/ml]. Western blot method showed that the levels of IL-6 in GDL and GDS（0.296±0.016、0.435±0.015）were significantly decreased compared with GM（0.455±0.026）.GDL was significantly lower than GA（0.426±0.012）. Conclusion Diosgenin can reduce the levels of serum and arteries IL-6 in the experiment atherosclerosis rats.