糖基化终末产物对人内皮细胞分泌前列环素和内皮素-1的影响

Influence of advanced glycation end products on prostacyclin and endothelin-1 secretion from human vascular endothelial cells

目的了解糖基化终末产物(AGES)对人内皮细胞分泌前列环素(PGI2)和内皮素-1(ET-1)的影响,以及α-硫辛酸(α-LPA)对其的影响。方法采用酶消化法收集人脐静脉内皮细胞,经原代培养后随机分为4组:正常对照组、AGES组、正常+AGES组和AGES+α-LPA组;放免法测定培养24、48、72 h时培养基中的PGI2和ET-1含量。结果①AGES组较正常对照组PGI2显著下降(P<0.05),ET-1显著升高(P<0.05);②AGES+α-LPA组较AGES组PGI2显著升高(P<0.05),ET-1显著下降(P<0.01)。结论 AGES对血管内皮细胞有损伤作用,α-LPA可减轻这种损伤,提示抗氧化剂可用于保护血管内皮细胞。

Objective To investigate the influence of advanced glycation end products（AGES） on prostacyclin（PGI2） and endothelin-1（ET-1） secretion from human umbilical vein endothelial cells（HUVEC）,and the effect of α-lipoic acid（α-LPA） on it.Methods HUVEC were obtained by enzyme digestion and primary culture,and divided into the normal control group,the AGES group,the normal＋AGES group,and the AGES＋α-LPA group.PGI2 and ET-1 in culture media were assayed by radioimmunoassay（RIA） when HUVEC were cultured for 24,48 and 72 hours. Results ①The content of PGI2 in the AGES group was lower than that in the normal control group（P0.05）,while the content of ET-1 was higher（P0.05）.②The content of PGI2 in the AGES＋α-LPA group was higher than that in the AGES group（P0.05）,while the content of ET-1 was lower（P0.01）.Conclusion AGES can injure vascular endothelial cells,while α-LPA can diminish the injury,which indicates that antioxidants can protect vascular endothelial cells.