cyclin D1蛋白在子宫内膜癌组织中的表达及其临床意义

Expression of cyclin D1 protein in endometrial carcinoma and its clinical significance

目的探讨cyclin D1蛋白在子宫内膜癌组织中的表达及其临床意义。方法采用免疫组织化学SP法检测20例正常子宫内膜、38例不典型增生子宫内膜及126例子宫内膜癌组织中cyclin D1蛋白的表达,分析其与子宫内膜癌临床病理因素的关系及对生存率的影响。结果 cyclin D1蛋白表达阳性率及表达强度在正常子宫内膜、不典型增生子宫内膜及子宫内膜癌中呈明显上升趋势,三者之间cyclin D1蛋白表达存在统计学差异(P<0.001)。cyclin D1蛋白表达阳性率及表达强度在Ⅲ～Ⅳ期病例明显高于Ⅰ～Ⅱ期(P=0.006);cyclin D1蛋白在组织学分级G1、G2、G3中的表达逐渐增加(P=0.010);淋巴结转移阳性者明显高于阴性者(P=0.008);Ⅱ型子宫内膜癌明显高于Ⅰ型(P=0.020)。cyclin D1蛋白表达与肌层浸润程度、腹腔液细胞学及患者年龄均无统计学相关(P>0.05)。cyclin D1蛋白阳性组患者的总体生存率明显低于阴性组患者(P=0.017)。结论 cyclin D1蛋白的过表达参与了子宫内膜癌的发生发展,提示其可作为有效的预后因子。

Objective To investigate expression of cyclin D1 protein in endometrial carcinoma and to evaluate its clinical significance.Methods Expression of cyclin D1 protein was determined by using the immunohistochemical method in 20 normal endometria,38 endometrial atypical hyperplasias and 126 endometrial carcinoms.The correlations of cyclin D1 expression with clinicopathological parameters and overall survival in endometrial carcinoma were studied.Results Immunohistochemistry showed that normal endometria and endometrial atypical hyperplasias were negligible for cyclin D1 expression.Expression of cyclin D1 in normal endometria and endometrial atypical hyperplasias was significantly less than that in endometrial carcinomas（P0.001）.Cyclin D1 expression was significantly associated with clinicopathological parameters,including surgical stage（P=0.006）,histological grade（P=0.010）,lymph node metastasis（P=0.008） and histological type（P=0.020）,but not with depth of myometrial invasion,positive peritoneal cytology and age（P0.05）.Patients with cyclin D1 expression had significant poorer overall survival compared with the patients without expression（P=0.017）.Conclusions Cyclin D1 overexpression may be involved in tumorigenesis and development of human endometrial carcinoma,which could be used as a predictor of the prognosis for the patients with endometrial carcinoma.