不同营养方式对普通小球藻生长特性和细胞组成的影响

The Effects of Different Nutrition Modes on Growth Characteristics and Cell Components of Chlorella vulgaris

采用自养、异养和混合培养3种营养方式对普通小球藻进行了培养,分析了3种培养方式下小球藻的生长曲线、比生长速率和产率、培养基中pH和葡萄糖的变化,测定了藻细胞的光合色素、蛋白质和油脂含量及其产率。结果表明:混合营养条件下小球藻的比生长速率为自养的4.25～4.43倍、异养的0.78～1.00倍,产率为自养的5.79～6.27倍、异养的1.11～1.31倍;混合营养条件下,单位体积小球藻培养液中总叶绿素、蛋白质和油脂的产率分别为3.62 mg/(L·d)、53.41 mg/(L·d)和44.65 mg/(L·d),均高于自养和异养培养。研究认为,混合营养方式更加符合微藻生长的实际环境,是高密度、高含量活性物质培养微藻的理想方式,具有潜在的应用价值。

The growth curves, specific growth rates and productivities of Chlorella vulgaris were analyzed under autotrophic, heterotrophic and mixotrophic cultivation, as well as the changes of pH values and glucose consumption in medium, the contents and productivities of photosynthetic pigment, protein and lipid were investigated. The results indicated that the specific growth rate of C. vulgaris under mixotrophic was to 4.25 -4.43times than autotrophic and 0.78 - 1.00 times than heterotrophic, the productivity of C. vulgaris in mixotrophic was to 5.79 - 6.27 times than autotrophic, and 1.11 - 1.31 times than heterotrophic cultivation. The values of C. vulgaris productivities for photo- synthetic pigment （3.62 mg! L. d）, protein （53.41 mg/ L. d） and lipid （44.65 mg/ L. d） were obtained under mixotrophic cultivation in per liters culture liquid, all kinds of value more than autotrophic and heterotrophic. The present paper showed that the mixotrophy be more fit for the physical environments of the microalgae growth conditions, and be suited to microalgae cultivation with high density and high content of bioactive substances.