摘要
Sialylation of glycoproteins is vital for the function or physicochemical properties of a protein. It becomes more and more important to develop approaches that can be used to efficiently isolate and identify sialylated glycopep- tides or glycoproteins for monitoring changes in glycoproteome. In the present study, we analyze intact structures of the enriched sialylated glycopeptides of bovine fetuin by matrix-assisted laser desorption/ionization-tandem mass spectrometry （MALDI-MS/MS）, without any chemical derivation. The experimental data show that the optimal loading buffer for TiO2 as matrix is 80% acetonitrile/2% TFA （trifluoroacetic acid）/100 mg/mL DHB （2,5-dihydroxybenzoic acid） which is also compatible with MALDI-mass spectrometric analysis. This study indi- cates that the improved enrichment approach combined with MALDI-MS/MS may be a powerful tool to analyze intact structures and components of the sialylated glycopeptides from complex peptide mixture.
Sialylation of glycoproteins is vital for the function or physicochemical properties of a protein. It becomes more and more important to develop approaches that can be used to efficiently isolate and identify sialylated glycopep- tides or glycoproteins for monitoring changes in glycoproteome. In the present study, we analyze intact structures of the enriched sialylated glycopeptides of bovine fetuin by matrix-assisted laser desorption/ionization-tandem mass spectrometry （MALDI-MS/MS）, without any chemical derivation. The experimental data show that the optimal loading buffer for TiO2 as matrix is 80% acetonitrile/2% TFA （trifluoroacetic acid）/100 mg/mL DHB （2,5-dihydroxybenzoic acid） which is also compatible with MALDI-mass spectrometric analysis. This study indi- cates that the improved enrichment approach combined with MALDI-MS/MS may be a powerful tool to analyze intact structures and components of the sialylated glycopeptides from complex peptide mixture.
基金
Project supported by National High-Technology Research and Development Program of China （No. 2006AA02Z154） and the National Natural Science Foundation of China （No. 21075137）.
