内皮祖细胞对狭长窄蒂皮瓣成活面积的影响

Study on the Survival Area of Narrow Pedicle Flap after Transplanting Endothelial Progenitor Cells

目的研究大鼠骨髓来源的内皮祖细胞(EPC)对狭长窄蒂皮瓣成活面积的影响。方法 SD大鼠15只,12只大鼠背部两侧各设计制作1块皮瓣,皮瓣设计为:蒂宽=蒂长=0.8 cm,蒂部所携带直径为3.5 cm的皮瓣,形状类似"乒乓球拍",建立皮瓣的动物模型,随机分为两组:对照组及实验组;另3只大鼠,冲洗骨髓腔,密度离心法获得单个核细胞;在适宜的培养环境中(EGM-2MV培养液)培养出EPC;鉴定细胞CD34、CD133、vWF及VEGFR-2表型,并将目的细注射移植于皮瓣,对实验组及对照组皮瓣进行大体观察,测量皮瓣成活面积,计算局部毛细血管密度。结果注射EPC的皮瓣的存活面积以及毛细血管密度显著高于对照部位(P<0.05)。结论 EPC移植于狭长窄蒂皮瓣后,加速皮瓣缺血缺氧组织再血管化,改善皮瓣缺血缺氧状态,扩大皮瓣成活面积。

Objective To study the effect of transplanting rat bone marrow derived endothelial progenitor cells to the survival area of narrow pedicle flap.Methods 12 SD rats were divided into 2 groups in random.A flap was designed and put on either side of a rat＇s back,and the flap was designed in the shape of a table tennis.The width and the length of the flap were both 0.8 cm and the pedicle was with the diameter of 3.5 cm.Bone marrow mononuclear cells were harvested by flushing bone marrow cavity from the other 3 SD rats and were isolated by density gradient centrifugation,cultured in endothelial growth medium-2.Identify the phenotype of CD34,VEGFR-2,vWF and CD133 of the isolated cells.Cells were injected into the skin flap.Gross observation and capillary vessel density evaluation was conducted.SD rats with culture fluid injection after skin flap implant were served as controls.Results The survival area and capillary density were more favorable in the EPCs injection sites than the controls（P0.05）.Conclusion Injection of enriched EPCs can significantly promote angiogenesis of the ischemia and hypoxia tissue,and advance the survival area of narrow pedicle flap.