左旋咪唑在新城疫疫苗接种鸡中免疫调节作用的研究

STUDY ON EFFECT OF IMMUNE REGULATION OF LEVAMTSOLE IN CHICKEN AFTER INOCULATING NEWCASTLE DISEASE (ND) VACCINE

本研究采用70 只具有较高母源抗体的3 日龄雏鸡,随机分为试验组和对照组。两组雏鸡均滴鼻接种鸡新城疫活疫苗( Ⅱ系)( 简称NDⅡ系苗)1 只份。从接苗的当日开始,在试验组雏鸡饲料内按每天每公斤体重15mg 计算,拌入盐酸左旋咪唑,连服3 日;对照组不服左旋咪唑。于接苗后的10 、20 、30 、40 和50 天,从两组雏鸡中随机各取30 只由翅静脉采血,析出血清,供测定血球凝集抑制(HI) 抗体效价用。同时,从各组鸡血样中随机各取10 份,作成全血涂片,供检测T淋巴细胞用。试验结果:试验组HI抗体效价稍高于对照组,二者差异不显著(P>0 .05) 。试验组T淋巴细胞百分率,在试验期间均高于对照组,二者差异极显著(P< 0.01)。由此可见,对具有较高母源体的雏鸡,在用NDⅡ系苗接种的同时,服用左旋咪唑,可以促进T淋巴细胞的增殖,增强其免疫力。

The 70 chickens which have higher maternal antibody, after inocubation 3 days, were randomly divided into two groups (exerimental group and check group), both of them was inoculated by ND line vaccine II at routine dosage. The checkens of experimental group were fed feed mixed levamisole hydrochloride 15mg/kg every ady for tree days. The feed for chickens of check group not mixed levamisole. After inoculating 10, 20, 30, 40 and 50 days, 30 chickens taken from two groups were collected blood respectively, to segregate serum, for testing hemagglutination in hibition antibody titer. In the same time, 10 portions of blood sample randomly taken from each group made blood smear respectively for testing T lymphocyte. The experimental result shows that the HI antibody titer of experimental group slightly higher than of check group, the differences of two groups were nonsignificant(P>0.05). The percentage of T lymphocyle from each experimental group higher than from check group during experimental period, the differences of both achieved an extremely significant level(P<0.01). Therefore, the checkens inoculated by ND II vaccine have higher materal antibody titer, feeding levamisole can promote proliferation of T lymphocyte, and strengthen its immunity.