结肠直肠肿瘤筛检方法初探

A for scanning method detecling colorectal tumors

目的 研究结肠直肠肿瘤人群筛检的方法。 方法 结肠直肠癌患者组 76例 ,结肠直肠息肉组患者 15例 ,对照组 15 9例 ,分别以肛指检查取得直肠粘液 ,与辣根过氧化酶 (HRP)标记的花生凝集素 (PNA)反应 ,以检测T抗原 ,呈棕色者为阳性标本 ,进行对比研究 ,其中 3 6例结肠直肠癌患者行免疫组织化学染色。 结果 PNA HRP检测结肠直肠癌的敏感性为 88 2 % ,特异性为 92 5 %。 结论 PNA HRP方法可用于结肠直肠肿瘤的人群筛检。

Objective To develop a method for survey of colorectal tumors. Methods Mucus obtained by digital rectal examination was smeared on a slice to react with peanut agglutinin labeled by Horseradish peroxidase. Results Brown color was noted in positive reaction. In detecting colorectal tumors, the sensitivity and specificity of the method was 88 2% and 92 5% respectively. Conclusion The method is effective in detecting colorectal tumors in humans.