端粒酶蛋白催化亚基(hTRT)mRNA在口腔鳞癌形成过程中的表达

Telomerase hTRT mRNA Expression in Oral Squamous Cell Carcinogenesis

目的:观察端粒酶催化蛋白基因hTRT在口腔粘膜恶性转化不同阶段中的表达,探讨其与口腔粘膜细胞恶性程度的关系。方法:用原位杂交技术检测82例标本,其中正常口腔粘膜7例、上皮单纯性增生7例、上皮异常增生30例、原位癌8例、口腔粘膜鳞癌30例。结果:正常口腔粘膜、上皮单纯性增生组织中hTRT的mRNA表达较弱,阳性信号仅局限于上皮基底层及副基底层间,阳性率21.4%(3/14);上皮异常增生中hTRTmRNA阳性表达见于多层上皮细胞,并随细胞异形性增高而表达增强,阳性率46.7%(14/30);口腔粘膜鳞癌组织中hTRT的mRNA有较强阳性表达,阳性率81.6%(31/38)。结论:端粒酶hTRT基因的表达与口腔粘膜细胞的恶性程度密切相关。

Objective: To investigate the relationship between telomerase hTRT gene expression and malignant development of oral mucosa epithlium. Methods: 82 cases were detected by in situ hybridization technique for hTRT gene, including 7 cases of normal oral mucosa, 7 cases of hyperplasia lesions, 30 cases of oral precancerous lesions (dysplasia lesion), 8 cases of oral mucosa carcinoma in situ and 30 cases of oral squamous cell carcinomas. Results: ①Weaker expression of hTRT mRNA was observed in normal oral mucosa and hyperplasia lesions. Positive cells were present in basal layer and parabasal layer, and hTRT positivity was 21.4% (3 of 14). ②The expression of hTRT mRNA in the precancerous lesions was gradually strong associated with phenotypic progression and the degree of dysplasia. Positive cell extended to multi layered epithelium, and hTRT positivity was 46.7% (14 of 30). ③Strong hTRT mRNA expression was observed in squamous cell carcinoma, and the positivity was 81.6% (31 of 38). Conclusion: Telomerase hTRT gene expression is closely related to the malignant degree of oral mucosa, the reactivated telo merase may play a crucial role in the progression of oral cancer.\;