肺炎衣原体对不同细胞株的敏感性研究

THE COMPARISON OF CHLAMYDIA PNEUMONIAE INFECTIVITY FOR DIFFERENT CELL LINES

目的 肺炎衣原体（Ｃｐｎ）是继鹦鹉热衣原体、沙眼衣原体之后鉴定的第三种衣原体。血清学研究表明，大部分人群均感染过Ｃｐｎ。它还和多种疾病有关，尤其是最近人们注意到Ｃｐｎ 与动脉粥样硬化（ＡＳ）之间存在密切关系。明确这种病原学关系对更好地明了ＡＳ发病机理，及对ＡＳ的诊断与治疗的研究至关重要。Ｃｐｎ 的分离和培养比其它衣原体困难，因此，选择一种合适的宿主细胞是关键所在。方法和结果 本文选择了ＨＬ，Ｈｅｐ－ ２ 和ＭｃＣｏｙ 三种细胞作比较；Ｃｐｎ 则选用ＴＷ－ １８３和ＡＲ－ ３９ 原型株。经培养后计算包涵体形成单位（ＩＦＵ），以比较各种细胞敏感性的差别。结论 经Ｓｔｕｄｅｎｔ－ Ｎｅｗ － Ｋｅｕｌ分析，发现Ｈｅｐ－ ２细胞是最适合繁殖Ｃｐｎ

Aim\ Chlamydia pneumoniae(Cpn) is the third strain differentiated after Chlamydia psittaci and Chlamydia trachomatis.Serology surveys suggested most population of adults were infected by Cpn.It is associated with many diseases.More particularly,Cpn has been associated with atherosclerosis(AS) recently.Thus,confirming this relation must play a significant role in understanding the mechanism of AS and treating the AS.But isolation and propagation of Cpn is more difficult than other two Chlamydiaes.In this respect,it is important to select a proper cell line.The study aim at the selecting a optimal cell line for the isolation and propagation of Cpn.Methods and Results\ Three cells were compared,that is,HL,Hep-2 and McCoy cell lines.Two type strains(TW-183 and AR-39)of Cpn were selected.Infectivity of cell lines was assessed for counting the number inclusion per microscopic field.Conclusions\ The result of Student-New-Keul analysis suggested that Hep-2 is superior to other two cell lines for propagating Cpn.