氧化苦参碱对乙型肝炎病毒转基因小鼠乙肝抗原表达的影响

Oxymatrine downregulate HBV gene expression in HBV transgenic mice

目的：研究氧化苦参碱抗乙型肝炎病毒的作用，并初步探讨其作用机制。方法：以乙型肝炎病毒全基因组转基因小鼠ＩＣＲ－ＴｇＮ（ＨＢＶａｄｒ１．２）ＳＭＭＵ 为动物模型，运用ＥＬＩＳＡ和免疫组化的方法检测小鼠肝脏内乙肝抗原含量。结果：分别予氧化苦参碱１００， ２００， ３００ ｍ ｇ／ｋｇ 腹腔注射，１次／ｄ，３０ ｄ 后，乙型肝炎病毒转基因小鼠肝脏内ＨＢｓＡｇ 和ＨＢｃＡｇ 的含量较空白对照组（予等体积生理盐水腹腔注射）均有明显的下降，且对两者作用一致；进一步的研究表明氧化苦参碱２００ ｍ ｇ／ｋｇ 作用１０ ｄ，２０ ｄ 时小鼠肝脏内ＨＢｓＡｇ 和ＨＢｃＡｇ 的含量较治疗前有明显的下降。结论：氧化苦参碱能降低乙型肝炎病毒转基因小鼠肝脏内ＨＢｓＡｇ 和ＨＢｃＡｇ 的含量，有抗乙型肝炎病毒作用。

Objective: To explore the effect of oxymatrine (Oxy) on HBV. Methods: HBV transgenic mice ICR TgN (HBV adr 1.2)SMMU was used in this study. ELISA and immunohistochemistry methods were adopted to determine the expression level of HBV in liver of transgenic mice. Results: Injected with Oxy 100, 200 or 300 mg/kg intraperitonealy once a day for 30 days later, HBV transgenic mice showed lower HBV gene product content in liver than control (injected with saline for 30 days). Further study indicated that Oxy can downregulate HBV gene expression when injected 200 mg/kg for 10 and 20 days. Conclusion: Oxy can decrease the content of HBsAg and HBcAg in HBV transgenic mice liver at the same time.