不同提取工艺下甘草提取物中甘草酸铵含量测定研究

Study on content determination of Monoammonium Glycyrrhizinate in the distilled licorice by different extraction technique

目的:建立甘草提取物中甘草酸铵的含量测定方法,优选甘草酸铵的提取工艺。方法:以甘草酸铵为指标,采用高效液相色谱法测定甘草酸铵含量;比较煎煮法、水提醇沉法、0.5%氨水提取与70%乙醇超声法等工艺的差异。结果:0.5%氨水提取甘草酸铵含量较高,甘草酸铵含量在5.2675～337.12μg范围里呈较好的线性关系;甘草酸铵的回收率为98.51%。结论:0.5%氨水提取工艺所得甘草提取物中甘草酸铵含量较高,甘草酸铵转移率最高,提取方法合理、可行、效率高。

Objective： To establish a method for content determination of Monoammonium Glycyrrhizinate in the distilled licorice and to optimize the extraction technique. Methods： Taking Monoammonium Glycyrrhizinate as index, we used the technique of HPLC to determine its content. Then compared the difference among water-decocting water-extracting and alcohol-depositing; 0.5% ammonia-extracting and 70% alcohol ultrasonic-method. Results： The content from 0.5% ammo- nia-extracting extraction of Monoammonium Glycyrrhizinate was the higher. In the range of 5.267 5 -337.12 μg, the Monoammonium Glycyrrhizinate showed good linear relationship. The recovery of Monoammonium Glycyrrhizinate was 98.51% . Conclusion： The content from 0.5% ammoniaextracting extraction of Monoammonium Glycyrrhizinate is the higher. The transfer rate of Monoammonium Glycyrrhizinate is the highest. The 0.5% ammonia -extracting is reasonable, feasible and efficient.