石墨烯/纳米金复合材料的无酶葡萄糖生物传感器制备

Investigation of Non-enzymatic Glucose Biosensor Based on Graphene/Gold Nanocomposites

以抗坏血酸(AA)为还原剂,通过同步还原法制得石墨烯/纳米金复合材料。采用电化学方法,构建了一种基于石墨烯/纳米金复合材料修饰电极的无酶葡萄糖生物传感器。实验中,通过伏安法考察了不同修饰电极在葡萄糖溶液中的电化学行为。同时,探讨了溶液中OH-离子强度、溶解氧、扫描初始电位及石墨烯与纳米金的比例对传感器响应特性的影响。在优化实验条件下,采用线性扫描伏安法检测葡萄糖的线性范围为0.1～20 mmol/L,检出限为1.6×10-5 mol/L(S/N=3)。对1 mmol/L葡萄糖平行测定10次,其相对标准偏差为2.7%。实验结果表明,此传感器具有较高的灵敏度、较好的重现性、稳定性及抗干扰能力。本方法可用于人血清样品中葡萄糖含量的测定,回收率为96.2%～103.2%,结果令人满意。

Graphene/gold nanoparticles（GNPs） nanocomposites were synthesized by a simple chemical reduction method.A novel non-enzymatic biosensor for glucose based on graphene/GNPs modified glassy carbon electrode was prepared by electrochemical method.The electrochemical behavior of different modified electrodes was investigated by cyclic voltammetry（CV）.Meanwhile,the effects of solution ion intensity,dissolved oxygen and initial scan potential on the sensor response characteristics were studied.Under the optimal conditions,the resulting biosensor displayed a rapid response to glucose.It shows a linear range from 0.1×10-3 to 20×10-3 mol/L with a detection limit of 1.6×10-5 mol/L（S/N=3）.The relative standard deviation was 2.7% for 1 mmol/L glucose（n=10）.The biosensor shows high sensitivity,good reproducibility,stability and can avoid the interference of commonly coexisted substances.The established method was successfully applied for the determination of glucose in human serum samples with the recovery of standard addition between 96.2% and 103.2%.