沙眼衣原体不同omp1基因型感染泌尿生殖道的临床特征研究

Clinical features of genitourinary tract infection with different omp1 genotypes of Chlamydia trachoamtis in Guangzhou

目的探讨泌尿生殖道沙眼衣原体(Ct)不同omp1基因型感染的临床特征。方法套式聚合酶链反应(Nested PCR)检测尿道(宫颈)拭子,诊断Ct omp1基因型感染,常规培养法检测泌尿道拭子中的淋球菌、解脲支原体/人型支原体,ELISA法检测单纯疱疹病毒,排除其他性传播感染。按照无症状感染、泌尿生殖道激惹症及异常分泌物三类临床表现,分析Ct各生物群、不同omp1基因型Ct感染的临床特征。结果共检测126例Ct阳性者,检出Ct 126株。宿主性别分析表明:三组生物群Ct中,各生物群Ct感染宿主时无性别选择性(P>0.05);所有感染者中,50%由B组感染,27.8%由C组感染,22.1%由中间组感染引起。对其中80例单纯Ct感染者的症状分析表明:无症状感染者中,女性多于男性(P<0.05);男性泌尿道感染omp1基因型D、F、J型常有症状(P=0.042<0.05);按生物组分析表明:B组、C组及中间组分别有50%、65.6%、55%感染者有症状,组间差异有统计学意义(χ2=8.67,P<0.05)。结论不同的Ct omp1基因型感染泌尿生殖道后,男女患者的症状和体征间有一定差异。

Objective To analyze clinical features of genitourinary tract infection with different omp1 genotypes Chlamydia trachoamtis（Ct） in Guangzhou.Methods Infections with different Ct omp1 genotypes were diagnosed by nested PCR and sequencing,and clinical features of distinct Ct omp1serovars and its B,C and intermediate bio-groups were also analyzed.Results Totally,126 cases were Ct positive,of whom no gender selections were observed for three Ct complex infections,and Ct B complex,C complex and intermediate groups contributed 50 %,27.8 % and 22.2%,respectively.Of the 126 cases,80（33 males and 47 females） were affected with single Ct infection;among asymptomatic cases,women were more than men（P〈0.05）.and men infected with serovar D/F/J were usually symptomatic.And 50.0%,65.6 % and 55.0% of cases in Ct B complex,C complex and intermediate groups had symptoms including abnormal urethral,cervical discharge or urethral irritant,and the difference was of statistical significance（P〈0.05）.Conclusion Ct genotype D,E,F and J were the most common genotype in Guangzhou,and the cases infected with different Ct genotypes had different symptoms and signs among men and women.