钝顶螺旋藻藻胆蛋白性质的研究

Properties of Phycobili-Proteins from Spirulina platensis

采用羟基磷灰石柱层析法对钝顶螺旋藻藻胆蛋白粗提液进行纯化,得到藻蓝蛋白(PC)和别藻蓝蛋白(APC)纯化液。用紫外-可见分光光度计测得 PC 和 APC 的最大可见吸收峰分别在620nm 和650nm 处;用荧光分光光度计测得它们的室温荧光发射峰分别在645nm 和656nm 处。经12%SDS-PAGE 法分析,PC由α和β两个亚基组成,其分子量分别为16.3kD 和18.9kD;APC 也由α和β两个亚基组成,其分子量分别为15.0kD 和16.9kD。用高效液相色谱仪(HPLC)对 PC 和 APC 的氨基酸组成作了测定,发现二者的氨基酸组成比较相似。

The purified phycocyanin(PC)and allophycocyanin(APC)were obtained from the phycobiliprotein crude extracts of Spirulina platensis by hydroxylapatite column chromatog- raphy analysis.The spectral characteristics of PC and APC were identified by ultraviolet-vis- ible spectrophotometer and spectrofluorimeter.The maximum visible absorption peaks of PC and APC were at 620 nm and 650 nm respectively,and the maximum fluorescence emission peaks of PC and APC at room temperature were at 645 nm and 656 nm respectively.The subunits of phycobiliproteins could be separated by 12% SDS-PAGE,which showed that PC consisted of two subunits:a(16.3 kD)and β(18.9 kD),APC also consisted of two sub- units:a(15.0 kD)and β(16.9 kD).The amino acid compositions of PC and APC showed by HPLC were very similar.