高效氯氰菊酯动式吸入染毒对大鼠的亚急性毒性试验

Sub-acute Toxicity Test of Dynamic Inhalation of Cypermethrin Microemulsion on Rats

目的建立农药连续动式染毒的方法,为开展农药毒理学2～4阶段试验创造必备条件。方法试验以液体农药(高效氯氰菊酯微乳剂)用2种浓度的稀释液进行雾化,在雾化过程中的3个时间段,于实验动物呼吸带水平设4个采样点,用多孔玻璃板吸收管采样气,用高效液相色谱仪测定定量,对染毒柜内的农药浓度进行测定。进而考察连续动式吸入染毒试验过程中农药的均匀性和稳定性。结果低剂量染毒组平均浓度为(99±24)mg/m3,高剂量染毒组平均浓度为(850±183)mg/m3。平均温度为(24.9±1.5)℃。平均湿度为(35.5±3.5)%。结论各个采样点平均浓度比较接近。表明染毒柜内低、高剂量毒物浓度的均匀性、稳定性比较好,达到连续动式吸入染毒标准要求。

[Objective]To establish the method for dynamic inhalation of pesticide on rats,and provide essential condition？for pesticide 2~4 phase experiment of toxicology. [Methods]The liquid pesticide（cypermethrin microemulsion） was atomized by diluent at two kinds of concentrations.The multiaperture glass plate absorbed tube was adopted to collect samples on 4 horizontal points of rats breathing zone during 3 period of atomization.The HPLC was adopted for quantitative determination of the pesticide concentration in cabinet. [Results]The mean concentration of low dose group was（99±24）mg/m3,that of high dose group was（850±183）mg/m3.The average temperature and average humidity was（24.9±1.5）℃ and（35.5±3.5）%,respectively. [Conclusion]The mean concentration of every sampling point is similar which indicates that the toxic concentrations of low-dose and high-dose are homogenized and steady and meet the requirements of dynamic inhalation standard.