人参细粉的鼠伤寒沙门菌基因回复突变试验

Effect of Panax Ginseng Powder on Salmonella Typhimurium Gene Reverse Mutation

目的体外观察人参细粉能否诱发鼠伤寒沙门菌组氨酸营养缺陷型突变株的回复突变。方法 Ames试验用TA97、TA98、TA100、TA102菌株并分加与不加肝微粒体酶活化系统(+/-S9)试验。结果人参细粉各剂量组对4个菌株的诱发回变菌落数都未超过自然回变菌落数,Ames试验结果为阴性。结论未见人参细粉对鼠伤寒沙门菌的致突变性。

[Objective]To test whether Panax ginseng powder can induce in vitro reverse mutation of Salmonella typhimurium histamine-auxotroph mutant. [Methods]Ames test,used TA97,TA98,TA100 and TA102 tester strains of Salmonella typhimurium with or without the addition of liver microsomal enzyme activation system（＋/-S9） was carried out. [Results]The number of Panax ginseng powder induced revertant colonies had no markedly difference with that of spontaneously revertant colonies with the 4 tester strains at a series of Panax ginseng powder doses,Therefore,the Ames test gave negative result for Panax ginseng powder. [Conclusion]Panax ginseng powder does not express mutagenesis.