胶原液晶膜的制备及对细胞生长的影响

Effects of Collagen Liquid Crystalline Films on Cell Culture

从仿生角度出发,制备了一系列液晶态胶原薄膜.采用偏光显微镜、扫描电镜和原子力显微镜分别研究了各种膜的液晶织构和拓扑结构,并通过3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法及细胞骨架荧光染色观察研究了各种膜对鼠成纤维细胞3T3的黏附、增殖以及形貌的影响.结果表明,胶原液晶薄膜呈现典型的胆甾型液晶织构,与非液晶薄膜相比,它不仅能更好地促进细胞的黏附、生长及增殖,而且其拓扑结构对细胞生长具有接触引导效应.

A series of biomimetic collagen films was prepared.The texture and topological structure of collagen films were studied by polarizing optical microscopy（POM）,scanning electron microscopy（SEM） and atomic force microscopy（AFM）.The relationship between collagen membranes and 3T3 cells was studied by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） method and cytoskeleton fluorescence staining.The results show that the collagen liquid crystal（LC） membranes present continuously twisting orientations in cholesteric phases characterized by typical series of arced patterns.Compared with the non-LC membranes,LC membranes not only could promote the cell＇s adhesion and proliferation,but also the LC topological structure had a contact guide effect to 3T3 cells.