树突形态在慢性脑缺血大鼠内侧前额叶的变化

Dendritic morphology of neurons in medial prefrontal cortex in chronic cerebral hypoperfusion rats

目的探讨慢性脑缺血大鼠模型内侧前额叶锥体细胞树突长度及树突棘密度的改变。方法将大鼠随机分为手术组及假手术组,手术组将双侧颈总动脉结扎。用高尔基染色法观察大鼠前额皮质区锥体细胞树突长度及树突棘密度。结果模型组大鼠内侧前额叶区树突棘密度较对照组低,树突长度无明显差异。结论慢性脑缺血大鼠内侧前额叶区树突棘密度减低可能是导致认知障碍的原因之一。

Objective To discuss the dendritic morphology changes of the pyramidal neurons of the medial prefrontal cortex（mPFC）after the chronic cerebral hypoperfusion in the 2VO rats.Methods The rats were divided into two groups：model group and shame operation group.The model rats were produced by the permanent occlusion of bilateral common carotid arteries.Then the brains were removed to study the dendritic morphology by Golgi stain method.Results The pyramidal neurons from the mPFC of the 2VO rats exhibited a reduction number of dendritic spines.But there were no significant differences of the dendritic length between the two groups.Conclusion The reduction of the dendritic density of the mPFC in the 2VO rats may be one of the causes leading to the cognitive disorder.