PPAR-γ2基因外显子B的P12A变异与2型糖尿病相关性研究

Relationship between pro12Ala polymorphisms in PPAR-γ2 gene and type 2 diabetes of littoral Han population in Zhejiang Province

目的研究过氧化物酶体增殖物激活受体-γ2（PPAR—γ2）基因外显子B的P12A变异与2型糖尿病（T2DM）及其相关临床特征。方法选取浙江沿海地区汉族人308例，其中T2DM组126例，对照组182例。对两组对象的基因型采用芯片分型方法进行检测；同时检测两组对象身高、体重、腰围、臀围、空腹血糖（FPG）、餐后2h血糖（2hPG）、血清胰岛素（FINS）及血脂；分析PPAR—γ2基因P12A变异的基因型与各临床变量的关系。结果T2DM组和对照组PPAR—γ2基因中P等位基因和A等位基因的分布频率分别是87．3％、89．6％和12．7％、10．4％，PP基因型频率是74．6％、79．1％，PA＋AA基因型频率是25．4％、20．9％，两组基因型及等位基因频率的差异均无统计学意义（均P〉0．05）。T2DM患者PPAR-γ2基因P12A变异携带者与腰围有关（P〈0．05）。结论PPAR—γ2基因P12A多态性在浙江沿海地区主要以PP基因型分布，T2DM组PPAR—γ2基因P12A变异与腰围有关，携带P12A基因者可能影响或加重T2DM脂代谢异常。

Objective To investigate the association of P12A variant in peroxisome proliferators activated receptor-γ2 （PPAR-γ2） gene with type2 diabetes. Methods The genotypes of P12A variants in PPAR-γ2 were determined by gene biochip assay in 308 subjects of littoral Hart population of Zhejiang Province, including 126 patients with type 2 diabetes metlitus, and 182 healthy subjects. The clinical characteristics including height, weight, waist circumference, hip circumference, fast plasma glucose （FPG）, 2h plasma glucose after glucose loading （2hPG） and fast insulin （FIN） were measured and their relationship with genotype of P12A variants was analyzed. Results The allele frequencies in the case and control group were 87.3%, 89.6% for P; 12.7%, 10.4% for A respectively. The genotype frequencies were 74.6%, 79.1% for PP; 25.4%, 20.9% for PA＋AA. The P12A variant of PPAR-γ2 was not significantly associated with type 2 diabetes （P 〉0.05 ）. The frequencies of the Ala 12 carrier in diabetic patients was associated with increased waist circumference（P〈0.05）. Conclusion The data show that P12A variant polymorphism of PPAR- γ2 in littoral Han population of Zhejiang is not associated with type 2 diabetes, but it might be a risk factor for metabolic disorder in diabetic patients.