亚甲基四氢叶酸还原酶基因多态性与原发性高血压患者合并冠心病的相关性研究

Association between MTHFR gene ploymorphism and primary hypertension complicated with CHD

目的探讨N5,10-亚甲基四氢叶酸还原酶(MTHFR)C677T位点突变与河南豫北地区原发性高血压及其合并冠心病发病的关系。方法选择原发性高血压患者405例为高血压组,高血压合并冠心病患者400例为冠心病组,健康体检者400例为对照组。对3组MTHFR基因C677T多态性进行基因分型。结果冠心病组T等位基因频率和TT基因型频率明显高于高血压组和对照组(P<0.05)。冠心病组TT基因型患者TC和血浆同型半胱氨酸水平明显高于CC+CT基因型(P<0.05)。结论 MTHFR基因C677T多态性与原发性高血压患者冠心病的发生相关。

Objective To investigate the association between the gene polymorphism of N5, 10-methylene tetrahydrofolate reductase （MTHFR） site-specific mulation C667T, primary hypertension and hypertension complicated with coronary heart disease（CHD） in Yubei area of Henan province,China. Methods In this case-control study, we selected 400 patients with primary hypertension complicated with CHD（CHD group）,405 patients with primary hypertension,and 400 controls. MTHFR C677T polymorphism was detected by PCR-RFLP. Results There was significant difference in allele frequency of three groups（P〈0.05）. The frequency of T allele and TT homozygotes in MTHFR in CHD group was higher than that in hypertension group and controls （P〈0.05）. The T allele and TT genotype increased the risk of CHD for patients with hypertension. The level of TC and plasma homocysteine in TT homozygotes was markedly higher than in CC or CT genotype in CHD group（P〈0.05）. Conclusions The pathogenesis of CHD in patients with primary hypertension may be associated with MTHFR genotypes,and the polymorphism of MTHFR C667T may play a role in occurrence of CHD in hypertensive patients.