新疆阿魏挥发油对吗啡依赖大鼠、小鼠戒断症状的作用

The effects of Ferula sinkiangensis essential oil on the withdrawal syndrome of Morphine dependent rats and mice

目的观察新疆阿魏挥发油(FSEO)对小鼠的镇痛作用以及对吗啡依赖大鼠、小鼠戒断症状的影响。方法采用扭体法、热板法观察FSEO对小鼠的镇痛作用,吗啡依赖大鼠、小鼠采用纳洛酮催促戒断,观察FSEO对戒断症状的影响。结果灌服不同剂量FSEO可不同程度的抑制化学刺激所致的小鼠扭体反应,提高热板致痛小鼠的痛阈值,扭体镇痛的ED50=3.26 g(生药量).kg-1。FSEO各剂量组对吗啡依赖大鼠催促戒断症状均有一定的抑制作用,还可明显抑制催促戒断所致的大鼠体重减轻,其体重下降与模型对照组比较有统计学。FSEO各剂量组对纳洛酮催促戒断后吗啡依赖小鼠的动物跳跃反应有一定程度的减少,而对催促戒断后小鼠的体重下降有较好的抑制作用,与模型对照组比较均有统计学差异。结论 FSEO对小鼠有一定的镇痛作用,对纳洛酮催促戒断后吗啡依赖大鼠、小鼠的体重下降和戒断症状有较好的抑制作用。提示FSEO作为戒毒新药有良好的研究价值和应用前景。

OBJECTIVE To evaluate the analgesic effects of Ferula sinkiangensis K.M.Shen essential oil（FSEO） on mice,and it＇s effects on the Morphine dependent SD rats and mice.METHODS The writhing reflex and hot plate test were used to evaluate the analgesic effects of FSEO on mice.The Morphine dependent SD rats and mice models were used to evaluate the effects of FSEO on the acute withdrawal syndromes.RESULTS The different doses of FSEO could inhibit the writhing response of mice caused by acetic acid in different degrees.FSEO could also raise the pain threshold of mice in hot plate test.The value of ED50 in writhing reflex was 3.26 g（crude drug）·kg-1.The middle dose（1.4 g·kg-1） of FSEO could significantly alleviate the acute withdrawal syndrome of rats compared with the NS group.At 45 min after the acute withdrawal the middle dose（1.4 g·kg-1） of FSEO could significantly alleviate the withdrawal syndrome（P0.01）.At 15 min and 45 min after the acute withdrawal,the low dose（0.7 g·kg-1） of FSEO could significantly alleviate the withdrawal syndrome of rats compared with the NS group（P0.01）.The tested doses could alleviate the Morphine dependent SD rats＇acute withdrawal syndrome.All the tested doses of FSEO could also alleviate the weight loss of Morphine dependent SD rats caused by acute withdrawal.This anti-weight loss effect was statistically different compared with the NS group（P0.01）.All doses of FSEO could alleviate the jumping reaction of Morphine dependent mice which was caused by acute withdrawal with Naloxone,but it was statistically insignificant compared to the model group.All doses of FSEO could significantly alleviate the weight loss of Morphine dependent mice after the acute withdrawal by Naloxone（P0.01）.CONCLUSION FSEO have analgesic effects on mice.FSEO could alleviate the withdrawal syndrome and weight loss reaction after the acute withdrawal by Naloxone in Morphine dependent mice and SD rats.Trial results suggests FSEO can be developed as a new anti-addiction drug.