摘要
为了对红球姜(Zingibe zerumbet)的染色体进行识别,并对该物种基因组的结构进行初步研究,利用PI和DAPI组合(CPD)染色与45SrDNA探针荧光原位杂交对中期染色体进行了分析。结果显示,红球姜具有2对45SrDNA位点,分别位于第5号和第10号染色体的短臂,对应于相应染色体上的显著CPD带区。基于rDNA位点和染色体测量数据,建立了红球姜准确而详细的分子细胞遗传学核型。
In order to identify the chromosomes of Zingibe zerumbet and preliminarily reveal its genome structure, CPD （combined PI and DAPI）staining and fluorescence in situ hybridization （FISH） with 45S rDNA probe were applied to analyze the mitotic metaphase chromosomes. Results showed that two pairs of 45S rDNA sites were respectively detected on the short arms of the chromosome 5 and 10, corresponding to the prominent red CPD bands. The molecular cytogenetic karyotypes of Zingibe zerumbet was constructed based on the data of rDNA and chromosome measurements.
出处
《畜牧与饲料科学》
2011年第11期11-13,共3页
Animal Husbandry and Feed Science
基金
湖南省教育厅一般项目(07C503)
关键词
红球姜
CPD染色
荧光原位杂交
核型
45S
RDNA
Zingibe r zerumbet
CPD staining
fluorescence in situ hybridization （FISH）
karyotype
45S rDNA
