摘要
为了探讨镍化物诱导BALB/c- 3T3 细胞转化过程中的分子毒作用, 本研究采用TRAP—银染法检测镍对转化细胞端粒酶活性的影响。结果显示, 硫化镍、氯化镍和硫酸镍在中等细胞毒性反应剂量水平诱导的BALB/c- 3T3细胞转化过程中的端粒酶活性均表现为阳性。提示, 镍对细胞端粒酶的阳性表达与细胞转化作用密切相关,
In orter to research the genotoxic lesions of nickel compounds inducing BALB/c-3T3 cell transformation,telomerase activities in these cells transformed were detected with TRAP-silver staining assay.The results showed that telomerase activities in BALB/c-3T3 cells transformed by nickel(Ⅱ) sulfide,nickel chloride and nickel sulfate were all positive,indicating that the positive expression of telomerase activity in the cells transformed might be related to genotoxic lesions and the cell transforming activity of three kinds of nickel compounds.These results demonstrate the carcinogenesis or possible carcinogenesis of these nickel compounds.
出处
《现代预防医学》
CAS
1999年第4期427-428,共2页
Modern Preventive Medicine
基金
国家自然科学基金!资助 (基金编号39170651)
