FQ-PCR法检测乙肝患者血清和精液中HBV DNA含量的临床意义

The clinical significance of testing HBV DNA in semen and serum with FQ-PCR

目的探讨应用荧光定量聚合酶链反应(FQ-PCR)法检测男性乙肝患者血清和精液中HBV DNA含量的临床意义。方法选取男性乙肝患者70例,分别采集患者的精液和静脉血,并用FQ-PCR法检测精液及血清中HBV DNA含量。结果精液标本中HBV DNA阳性16例(占22.86%),HBV DNA含量为2.55×103～6.18×105IU/ml、平均为5.08×104IU/ml;血清标本中HBV DNA阳性62例(占88.57%),HBV DNA含量为1.21×103～7.52×107IU/ml、平均为9.40×105/ml。精液中HBV DNA含量与血清中HBV DNA含量呈正相关(r=0.743),且血清HBV DNA含量高于精液含量10倍左右;结论 FQ-PCR法定量检测精液中HBV DNA含量具有较强的特异性和灵敏度,为临床了解乙肝患者精液中HBV状态提供了帮助,具有重要的临床意义。

Objective To evaluate the clinical value of FQ-PCR for testing HBV DNA in semen and serum of male HBV. Methods HBV DNA in semen and serums of 70 males were tested with FQ-PCR. Results There were 16 positive samples of HBV DNA in semen samples,and the positive rate was 22.86%. The IU number was 2. 55× 10^3 - 6.18 × 10^5/ml, and the average number was 5.08 × 10^4/ml. There were 62 positive samples of HBV DNA in 70 serums samples,and the positive rate was 88.57%. The IU number was 1.21×10^3-7.52×10^7/ml,and the average number was 9. 40×10^5/ml. Conclusions FQ-PCR showed high specificity and sensitivity,which bad important clinical significance to evaluate the situation of HBV DNA viruses in semen samples.