HPLC法同时测定双黄连片中黄芩苷、绿原酸、连翘苷、连翘酯苷的含量

Content Determination of Baicalin,Chlorogenic Acid,Forsythin and Forsythoside A in Shuanghuanglian Tablets by HPLC

目的:建立同时测定双黄连片中黄芩苷、绿原酸、连翘苷、连翘酯苷含量的方法。方法:采用高效液相色谱法。色谱柱为Hedera ODS 2柱,流动相为甲醇-0.2%磷酸溶液,采用梯度洗脱,流速为1.0mL.min-1,检测波长为324、280nm,进样量为10μL,柱温为30℃。结果:黄芩苷、绿原酸、连翘苷、连翘酯苷检测浓度分别在28.525～342.3μg.mL-1(r=0.9999)、4.56～54.72μg.mL-1(r=0.9995)、1.55～18.6μg.mL-1(r=0.9994)、1.81～21.72μg.mL-1(r=0.9996)范围内与峰面积积分值线性关系良好;4种成分平均加样回收率分别为98.12%、96.99%、103.16%、100.14%(n=6),均符合含量测定要求。结论:本法简单易行,可用于双黄连片的质量控制。

To establish a method for simultaneously determination of baicalin, chlorogenic acid, forsythin and forsythoside A in Shuanghuanglian tablets. METHODS： HPLC method was carried out on a Hedera ODS 2 column with mobile phase consisted of methanol-0.2 % phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL. rain ~. The detection wave- length was 324 nm and 280 nm. Injection volume was 10μL and column temperature was 30 ℃.RESULTS： The linear ranges of baicalin, chlorogenic acid, forsythin, Forsythosid A were in the range of 28.525～342.3 μg.mL^-1（r=0.999 9）, 4.5654.72 pg.mL-l（r=0.999 5）, 1.55～18.6 lag.mL 1（r=0.999 4）, 1.81～21.72 p.g.mL^-1（r=0.999 6）, respectively. The average recoveries of four active ingredients were 98.12%, 96.99%, 103.16%, 100.14%, respectively （n=6）. CONCLUSION： The method is sim- ple, feasible and very suitable for the quality control of Shuanghuanglian tablets.