肿瘤坏死因子β诱导淋巴细胞化学发光及其增殖的研究

Study on the chemiluminescence and proliferation of lymphocyte induced by rhTNFβ

目的：探讨活性氧参与ｒｈＴＮＦβ诱导淋巴细胞活化作用的机理。方法：采用化学发光（ＣＬ）和顺磁共振法（ＥＳＲ）研究ｒｈＴＮＦＢ诱导人外周血淋巴细胞产生活性氧（ＯＲ）的动态变化及其增殖作用。结果：①用１０－２０００Ｕ／ｍｌ的ｒｈＴＮＦβ处理１×１０６ｍｌ－１淋巴细胞均可诱发Ｌｙ－ＣＬ，其中以１００Ｕ／ｍｌ ｒｈＴＮＦβ处理１８～２０ｍｉｎ的ＣＬ峰值（ＲＬＩ）为最高，所诱发的ＯＲ主要为ＯＨ·；②以１５～６２Ｕ／ｍｌ的ｒｈＴＮＦβ处理淋巴细胞７２ｈ，可明显地产生活化效应，这与ＴＮＦＢ诱发淋巴细胞早期产生Ｌｙ－ＣＬ的强度相一致。结论：适当剂量的ｒｈＴＮＦβ可诱导人外周血淋巴细胞产生ＯＲ并促进其增殖。

Objective :Study on the mechanism of lymphocyte's activation induced by rhTNFB with the Participation of active oxygenredicals (OR) .Methods:It was using chemiluminescnce (CL) and electron spin resonance (ESR ) techniques Results: 1. The lymphocytess (1 10~6 ml-1 )treated with 10~2000 U/ml rhTNFβ can induce CL.The highest peak value of CL(RLI)was Obtained at 100 U/nd rhTNFβ for 18~20 min.The induced OR was mainly OH.2.After the lymphocyte was treated with 15~62 U/ml rhTNFβ for 72 h,the proliferation effect wasobviously Observed. This effect was in agree with the CL intensity produced earlier.Conclusion: A suitable dose of rhTNFβ can induce OR Production of lymphocyte and promote its proliferation.