Pb(Ⅱ)和Cd(Ⅱ)在长根菇菌丝体中的富集及其对SOD、CAT酶活的影响

Enrichment of Pb(Ⅱ) and Cd(Ⅱ) in Oudenmansiella radicata mycelium and its effect on SOD and CAT enzyme activity

以长根菇菌丝体为材料，测定其对Pb（Ⅱ）和Cd（Ⅱ）的富集能力，以及不同浓度的Pb（Ⅱ）和Cd（Ⅱ）对菌丝体超氧化物歧化酶（SOD）和过氧化氢酶（CAT）酶活的影响．研究发现：低浓度Pb（Ⅱ）（100mg／L）明显刺激长根菇菌丝体的生长，而高浓度的Pb（Ⅱ）和Cd（Ⅱ）则抑制其生长；Pb（Ⅱ）和Cd（Ⅱ）的浓度越大，菌丝体对其富集能力越强，其最大富集量可分别达1．2132×10^4mg／kg（Pb（Ⅱ））和1．99872×10^3mg／kg（Cd（Ⅱ））．此外，研究还发现，Pb（Ⅱ）和Cd（Ⅱ）可提高菌丝抗氧化酶的活性，其CAT酶活可分别提高至1．131U／g（Pb（Ⅱ），100mg／L）和2．263U／g（Cd（Ⅱ），1mg／L）；其SOD酶活可分别提高至13．976U／g（Pb（Ⅱ），200mg／L）和14．668U／g（Cd（Ⅱ），2mg／L）．

OudenmansieⅡa radicata mycelium was chosen as the research material,and its enrichment capability of Pb（ Ⅱ ） and Cd（ Ⅱ ）, as well as the effect of its SOD and CAT enzyme activity under different concentrations of Pb（ Ⅱ ） and Cd（ Ⅱ ）, were studied. The results showed that, low concentration of Pb （ Ⅱ ） （100rag/L） significantly stimulated the growth of the mycelium, while high concentrations of Pb （ Ⅱ ） and Cd（ Ⅱ ） inhibited its growth. With the increasing of Pb（ Ⅱ ） and Cd（ Ⅱ ） concentration, the enrichment capability of the mycelium increased. The highest enrichment amount can reach 1. 2132×10^4 mg/kg for Pb（ Ⅱ ）and 1. 99872×10^3 mg/kg for Cd（ Ⅱ ）. Besides, this study also found that, Pb（ Ⅱ ） and Cd（ Ⅱ ）can increase the activity of antioxidant enzymes of Oudenmansiella radicata mycelium. Its CAT activity can reach up to 1. 131 U/g （Pb（ Ⅱ ）, 100 mg/L） and 2. 263 U/g （Cd（ Ⅱ ）, 1 rag/L）, respectively, and SOD activity can reach up to 13. 976 U/g （Pb（Ⅱ）, 200 mg/L） and 14. 668 U/g （Cd（Ⅱ）, 2 rag/ L）, respectively.