宫颈癌组织差异表达基因的检测及分析

The detection and analysis of differentially expressed genes in Cervical cancer tissues

选取三例经分型明确为HPV16感染的宫颈癌病例,取癌组织(T)、癌旁组织(N)及远瘤正常宫颈组织(F)标本,用Roche NimbleGen芯片系统筛选出差异表达的基因并进行分析.三例标本中,癌组织与远瘤正常宫颈组织相比,差异表达基因共有673个,其中261个上调,412个下调;差异表达基因主要有原癌基因、抑癌基因、细胞信号转导、代谢、免疫应答、细胞受体、蛋白翻译合成有关基因等.

The human gene expression of cervical cancers infected with HPV16 and their perienchyma were analyzed with Roche NimbleGen system. The analysis demonstrated that there are 673 differential expressed genes between cervical cancers and their normal cervical tissues （TvsF）. Compared to normal cervical tissues, the expression of 261 genes was up-regulated and expression of 412 genes was down- regulated in cervical cancers. All of the differential expressed genes involved in the signal transduction, metabolism, immunity response, protein biosynthesis and ESTs.