去甲斑蝥素致大鼠多脏器功能失常综合征的动态观察

A dynamic observation on multiple organ dysfunction syndrome caused by cantharidin in rats

目的：探讨多脏器功能失常综合征（ＭＯＤＳ）病程中重要脏器功能的变化以及与氧自由基的关系。方法：用ＳＤ大鼠制作ＭＯＤＳ模型，动态检测脏器功能及血清超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ Ｐｘ）和丙二醛（ＭＤＡ）的变化。结果：血清丙氨酸氨基转移酶（ＡＬＴ）、肌酸激酶及其同工酶（ＣＫ 和ＣＫ ＭＢ）和肌酐（Ｃｒ）均明显升高，其中ＣＫ ＭＢ于１２ 小时明显升高，１８ 小时达到高峰（Ｐ＜ ０．０１）；ＣＫ于１２小时最先达到衰竭标准（Ｐ＜ ０．０１）；ＡＬＴ与Ｃｒ均在２４ 小时后达到衰竭标准（Ｐ＜ ０．０５）。同时ＳＯＤ、ＧＳＨ Ｐｘ活性降低和ＭＤＡ含量增多（Ｐ均＜ ０．０５）。ＭＤＡ与ＣＫ、ＡＬＴ和Ｃｒ之间呈正相关关系，ｒ值分别为０．８４７、０．６３４和０．５６７（Ｐ均＜ ０．０１）。结论：在去甲斑蝥素致大鼠ＭＯＤＳ的病程中，心功能衰竭出现在先，然后为肾和肝功能衰竭。器官功能受损与氧自由基有关。

Objective:To investigate the changes in vital organ functions in the course of multiple organ dysfunction syndrome (MODS) and their relationship with oxygen free radicals.Methods:A rat model of MODS was made to assess organ functions at various intervals (6,12,18 and 24 hours),and changes in serum superoxide dismutase (SOD),glutathione peroxidase (GSHPx) and malondialdehype (MDA) were also measured.Results:A significant elevation of alanime transaminase (ALT),creatine kinase (CK) and creatinine (Cr) was observed,of which CK was the first parameter to fulfill failure standard ( P <0 01) at 12 hours,while ALT and Cr fulfilled the criteria at 24 hours (both P <0 05).Meanwhile,serum SOD and GSHPx decreased,and MDA contents increased (all P <0 05).In addition,significantly positive correlations were shown between MDA and CK( r =0 847),ALT( r =0 634),Cr( r =0 567),respectively(all P <0 01).Conclusions:In the course of MODS caused by cantharidin in rats,cardiac failure precedes the renal and hepatic failure.Organ dysfunction is associated with oxygen free radicals.