摘要
用超声波提取、薄层扫描法测定止血定痛片中人参皂甙Ｒｇ１ 的含量， 方法简便， 结果准确。薄层条件为： 含０－２％ ＣＭＣ－ Ｎａ 的硅胶Ｇ 板， ［ 正丁醇－ 乙酸乙酯－ 水（３∶２∶５）］ 上层液－ 甲醇（１０∶１） 为展开剂（ 展开缸预先用氨蒸汽饱和半小时） ， １０ ％ 硫酸乙醇加热显色。扫描波长λｓ ＝ ５３０ｎｍ ，λＲ＝ ７００ｎｍ ， 平均回收率为９６－３８ ％ ， ＲＳＤ 为１－０５ ％ 。
A simple, rapid and accurate TLC scanning method for the quantitative determination of Ginsenside-Rg 1 in Zhixue Dingtong tablet has been established. The sample was extracted by ultrasonic method. The plate was coated with silica gel G added 0 2% CMC-Na. Using upper mixture [n-butanol-ethyl acetate-water (3∶2∶5)]-methanol (10∶1) as developing solvent. The plate was scanned at λ s=530nm, λ R=700nm. The average recovery was 96 38% with a RSD of 1 05%
出处
《中国药事》
CAS
1999年第6期403-404,共2页
Chinese Pharmaceutical Affairs
