乌司他丁对急性肺损伤小鼠水通道蛋白1、5表达的影响

Effect of ulinastain on expression of aquaporins 1 and 5 in mice with acute lung injury

目的研究急性肺损伤(ALI)小鼠肺组织中水通道蛋白1(AQP1)、水通道蛋白5(AQP5)的表达变化,探讨乌司他丁(UTI)治疗ALI的机制。方法 32只昆明小鼠随机分为空白对照组(NS组)、造模组(ALI组)、乌司他丁疗组(UTI组)、地塞米松组(Dex组),观察6h后测定肺湿干重比值(W/D),并采用HE染色观察炎症变化,RT-PCR检测AQP1、AQP5、TNF-α、IL-1β mRNA的表达变化。结果乌司他丁及地塞米松治疗后,肺组织炎症、充血明显改善。与NS组相比,ALI组AQP1 mRNA的表达显著降低(P<0.05),与ALI组相比,UTI组、Dex组的AQP1 mRNA的表达显著升高(P<0.05)。AQP5 mRNA表达的变化与AQP1 mRNA的变化相似。与NS组相比,ALI组TNF-α mRNA的表达显著升高(P<0.05),与ALI组相比,UTI组、Dex组TNF-α mRNA的表达显著降低(P<0.05)。IL-1βmRNA表达的变化与TNF-αmRNA的变化相似。结论 UTI可通过上调AQP1、AQP5的表达减轻肺损伤时肺水肿。可能通过抑制炎症因子TNF-α、IL-1β的表达实现。

Objective To study the effect of ulinastain（UTI） on acute lung injury（ALI） by studying the expression of aquaporin 1（AQP1） and aquaporin 5（AQP5） in lung tissue of rats.Methods Thirty-two Kunming mice were randomly divided into control group,ALI group,UTI group and dexamathasone（Dex） group.Their lung wet/dry（W/D） mass ratio was assayed,inflammatory change was observed with HE staining,and expressions of AQP1mRNA,AQP5 mRNA,TNF-αmRNA and IL-1βmRNA were detected by RT-PCR after 6h.Results The inflammation and congestion in lung tissue were significantly improved after treatment with UTI and Dex.The expression level of AQP1mRNA was significantly lower in ALI group than in control group（P〈0.05）,and significantly higher in UTI and Dex groups than in ALI group（P〈0.05）.The expression level of TNF-αmRNA was significantly lower in UTI and Dex groups than in ALI group（P〈0.05）.No significant difference was observed in expression of IL-1βmRNA and TNF-αmRNA.Conclusion UTI can improve pulmonary edema during lung injury by up-regulating the expression of AQP1 and AQP5 and down-regulating the expression of TNF-αand IL-1β.