大黄素改善KKAy糖尿病小鼠胰岛素敏感性的实验研究

Emodin improves insulin sensitivity in KKAy diabetic mice

目的探讨大黄素改善KKAy糖尿病小鼠胰岛素抵抗的作用机制。方法将SPF级KKAy小鼠50只按血糖值随机分为模型组,大黄素低、中、高治疗组按12.5、25、50mg/(kg·d)剂量灌胃,吡格列酮治疗组按1.95mg/(kg·d)剂量灌胃,并选C57BL/6J小鼠为正常对照组,连续灌胃给药8周。8周后测定空腹血糖(FPG)、空腹胰岛素(Fins)并计算胰岛素敏感指数(ISI)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),游离脂肪酸(FFA),超敏C反应蛋白(hs-CRP)及肿瘤坏死因子-α(TNF-α)。结果大黄素可以降低KKAy糖尿病小鼠空腹血糖、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、FFA、hs-CRP及TNF-α,并能改善胰岛素敏感性。结论大黄素能降低血糖及肿瘤坏死因子-α并改善KKAy糖尿病小鼠的胰岛素敏感性。

Objective To study the effect of emodin on insulin resistance in KKAy diabetic mice and its underlying mechanism.Methods Fifty specific pathogen free（SPF） KKAy mice were randomly divided into low dose emodin group,medium dose emodin group,high dose emodin group,model group,and pioglitazone group according to their plasma glucose level.Mice in the first 4 groups received intra-gastric emodin（12.5,25,50mg/kg·d） and those in pioglitazone group received intra-gastric pioglitazone（1.95mg/kg·d） for 8 weeks.C57BL/6J mice served as a normal control group.Then,their fasting plasma glucose（FPG） and fasting insulin（Fins） levels were measured to calculate insulin sensitivity index（ISI）,total cholesterol（TC）,total triacylglycerol（TG）,high-density lipoprotein cholesterol（HDL-C）,low-density lipoprotein cholesterol（LDL-C）,floating fatty acid（FFA）,high sensitive C-reaction protein（hs-CRP）,and tumor necrosis factor-α（TNF-α） were measured,respectively.Results Emodin reduced the levels of FPG,TC,TG,LDL-C,FFA,hs-CRP and f TNF-α,and improved the insulin sensitivity in KKAy diabetic mice.Conclusion Emodin can reduce blood glucose and TNF-αlevels,and improve the insulin sensitivity in KKAy diabetic mice.