Eotaxin/CCR3在子宫内膜异位症不孕小鼠的表达及中药莪棱胶囊的干预作用

Eotaxin/CCR3 Expression in Endometriosis Infertility Mice and the Invention of Traditional Chinese Medicine Erling Capsules

目的:通过建立子宫内膜异位症(EMS)导致不孕症小鼠模型,观察比较趋化因子EOTAXIN及其受体CCR3在子宫内膜异位症(内异症)怀孕鼠以及不孕鼠之间的差异,分析EOTAXIN/CCR3基因对内异症不孕的影响,并探讨中药莪棱胶囊的干预作用。方法:建立EMS小鼠模型,同时皮下注射乙烯雌酚。观察24周后,进行同笼交配以计算该模型不孕率,同时区分怀孕鼠以及不孕鼠。对不孕鼠口服给予莪棱胶囊进行治疗,4周后取材,采用荧光定量PCR法分别观察正常鼠、怀孕鼠、不孕给药鼠、不孕给水鼠子宫内膜中Eotaxin/CCR3的表达情况。结果:假手术组小鼠子宫内膜Eotaxin表达,以及CCR3基因表达均较低。EMS模型怀孕鼠子宫内膜Eotaxin/CCR3基因表达明显升高。但EMS模型不孕鼠子宫内膜Eotaxin,以及CCR3基因表达较怀孕鼠更加明显。在不孕鼠中,给予莪棱胶囊的小鼠,子宫内膜Eotaxin表达以及CCR3表达均较给水组降低。结论:EOTAXIN/CCR3可能直接参与了子宫内膜异位症导致的不孕症的病理过程。莪棱胶囊可直接下调不孕鼠子宫内膜EOTAX-IN/CCR3表达,而起到缓解炎症,改善内异症作用的。

Objective： To establish endometriosis（EMS） infertility in mice models,observe and compare the difference of expression of chemokine EOTAXIN and its receptor CCR3 between endometriosize pregnant and infertile mice,analyze the effect of EOTAXIN/CCR3 gene expression on endometriosis infertility,and to explore Traditional Chinese Medicine Erling capsule intervention.Methods： Established an EMS mouse model,while subcutaneous injection of diethylstilbestrol.Observed after 24 weeks,mice were mated within the same cage,the rate of infertility was calculated and pregnant mice and infertile mice were distinguished.Infertile mice were given oral Erling capsules for treatment for 4 weeks,endometrial in each mouse was drawn and quantitative PCR was used to detect the difference expression of Eotaxin/CCR3 within normal mice,pregnant mice,and infertile mice treated with Erling capsules or not.Results： Eotaxin expression and CCR3 expression were lower in Sham group of mice.Endometrial Eotaxin/CCR3 gene expression was significantly increased in EMS pregnant mice.Eotaxin and CCR3 gene expression in EMS infertile mice is more obvious than in the pregnant mice endometrium.As for infertile mice,Eotaxin/CCR3 expression in mice given Erling capsule was decreased compared to the mice treated with water.Conclusion： EOTAXIN/CCR3 may be directly involved in the pathological process of infertility which caused by endometriosis.Erling capsules can relieve inflammation and improve endometriosis,the mechanisms may be related to directly down-regulated the expression of EOTAXIN/CCR3 in mouse endometrium.