血清CREG蛋白在急性心肌梗死早期的表达研究

The Expression of Serum CREG Protein in the Early Period of Acute Myocardial Infarction

目的:探讨血清中CREG蛋白在急性心肌梗死发作早期的表达情况,尝试为临床心肌缺血的极早期诊断提供一种新的血清标志分子。方法:在2010年6月至2010年11月期间,入选在沈阳军区总医院心内科住院治疗的急性ST段抬高型心肌梗死患者50例及非AMI对照50例,于AMI组胸痛发作后的不同时间点采血测定CK、CK-MB、LDH和cTnT,同时应用Western blot技术测定血清中CREG蛋白的含量,并与对照组比较。结果:AMI组发病72小时内的血清中CREG蛋白表达均较对照组有不同程度的增高(P<0.05)。胸痛开始2h内,AMI组血清中CREG的含量即明显增高,其在2h、4h及6h的含量显著高于对照组(P<0.001)。在胸痛已经发作2小时内,两组间血清cTnT、CK、CK-MB及LDH水平比较无统计学意义(P>0.05)。结论:CREG在AMI患者血清中的表达增高,其在血清中表达时间早于cTNT及CK-MB。

Objective： To investigate the early expression of CREG protein in serum of patients with acute myocardial infarction, and provide new＇ serum marker molecule for the very early clinical diagnosis of myocardial ischemia. Methods： In June 2010 to November 2010 period, 50 patients were selected,who hospitalized with acute ST-segment elevation myocardial infarction were selected and 50 non-AMI cases were taken as control, in the General Hospital of Shenyang Military Region, Department of Cardiology. CK, CK-MB, LDH and cTnT were detected at different time points after chest pain in the AMI group, serum CREG protein were tested by Western blot technique. Results：Within 72 hours of onset of AMI group, the serum CREG protein expression increased with different degrees compared with that in the control groups （P 〈0.05）. During the first 2 hours after chest pain, AMI serum levels in CREG increased signifi- cantly at the 2h, 4h and 6h levels （P 〈0.001）. There was onset of chest pain within 2 hours, cTnT, CK, CK-MB and LDH levels had no statistically significant difference between the two groups （P〉 0.05）. Conclusions： The expression of CREG in the serum increased in patients with AMI, and its expression in the serum was earlier than cTNT or CK-MB.