耳鼻咽喉真菌病的病原菌分离与鉴定研究

ISOLATION AND IDENTIFICATION OF PATHOGENIC FUNGI FOR OTORHINOLARYNGEAL MYCOSIS

目的：研究耳鼻咽喉真菌病的病原菌分离与鉴定。方法：取耳鼻咽喉真菌病病灶的分泌物、假膜、皮屑，病变组织涂片镜检，真菌阳性者同时做真菌培养以鉴定菌种。结果：２１９ 例涂片阳性及组织病理学诊断为真菌病中，１９９ 例分离出病原菌，阳性率９０ ．９ ％ 。耳鼻咽喉真菌病的主要原菌为曲霉菌，其次为念珠菌属，再次为青霉菌、尖端单孢子菌、孢子丝菌、卡氏枝孢霉、互隔交链孢霉；外耳道口及耳廓主要为皮肤癣菌。结论：病原菌的分离与鉴定，对临床上选用有效的抗真菌药有指导意义。

Objective: To isolate and identify the pathogenic fungi of otorhinolaryngeal mycosis. Methods: Direct smear and microscopical examination of secretory material, pseudomembrance, dermal scraps and tissues from fociwith otorhinolaryngeal infection were conducted. Fungus culture was performed in the fungi positive cases for microscopical identification. Results: Pathogenic fungi were isolated in 199 of the 219 microscopical examination positive or pathological diagnosis cases. The positive rate of culture was 90.9%. Pathogenic fungi of otorhinolaryngeal mycosis were chiefly Aspergillus, Candida albicans and Penicillium citrinum, Monospotium apiospermum, Sporothrix, Cladosporium carrionii and Alternaria alternate, and dermatophyte for infection of auricle and external acoustic meatus. Conclusion: The isolation and identification of pathogenic fungi of otorhinolarynologeal mycosis is greatly significant for the clinical selection of efficient antifungal drugs. [