CEA-重组痘苗病毒对CEA阳性肿瘤疗效的实验研究

The Therapeutic Effect of CEA Recombinant Vaccinia Virus on CEA Positive Tumor

目的:为探讨CEA-重组痘苗病毒对CEA阳性肿瘤的治疗作用.方法:构建CEA阳性小鼠Hepa肝癌细胞模型(Hepa-CEA);对4组实验鼠进行颈背部皮下注射CEA阳性或CEA阴性同源鼠Hepa肝癌细胞,再接种痘苗病毒(野生型W-VV或重组型CEA-rV)3次:观察动物反应,并在接种癌细胞后每周测量一次皮下肿瘤大小.结果:4组实验鼠颈背部皮下均可见瘤块形成,但3个对照组肿瘤生长迅速,组间差异无统计学意义(P>0.05),而接种Hepa-CEA/CEA-rV组小鼠皮下肿瘤生长缓慢,瘤块较小,与3个对照组比较有统计学上的显著差异(P<0.01),整个实验过程中接种鼠未表现有毒副反应,结论:CEA-rV可能通过诱导机体免疫系统产生CEA特异性的细胞或体液免疫应答反应,对CEA阳性肿瘤的生长明显产生抑制作用.

Objective: To study the therapeutic effect of CEA recombinant vaccinia virus on CEA positive tumor. Methords: The CEA positive cell model of murine Hepa liver cancer was constructed. Then, 40 mice, which were divided into four groups, were injected subcutaneously with CEA positive or CEA negative Hepa tumor cells respectively, and followed by subcutaneous vaccination of wild-type vaccinia virus (W-VV ) or CEA-rV for three times at 14-day interval . The animals responses were observed and the subcutaneous tumors were measured weekly after tumor cells injection. Results: Subcutaneous tumors were observed in all mice. Three control groups tumors grew relatively fast, no statistic significance between these groups were obtained ( P > 0.05), whereas the tumors in group four, which were injected with Hepa-CEA^(+) tumor cells and CEA-rV , grew slowly and the tumor mass was smaller. Comparison with the three control groups, the difference was significant in statistics ( P < 0.01). During the whole testing period, no toxic or side-effects were seen in all animals. Conclusion: The results showed that CEA-rV had well therapeutic effect on CEA positive tumors through inducing the body's immune system to produce CEA specific immune responses.