玉米P1811近缘系遗传多样性的SSR标记

Genetic Diversity of Maize P1811 Relatives with SSR Markers

为了研究P1811自交系的亲缘关系,利用SSR标记技术分析了77个玉米P1811近缘系的亲缘关系及遗传多样性。结果表明:25对多态性较好的随机引物中共检测到222个等位基因变异,每对引物检测等位基因4～16个,平均8.88个;SSR引物的PIC值介于0.615 7～0.938 1,平均为0.87;GS变幅为0.518 0～0.882 9,平均为0.708 6。利用UPGMA聚类分系法将供试自交系划分为7类,该划分结果与根据地理来源、种质系谱的分类结果基本一致。结论:77个玉米P1811近缘系材料之间具有一定的遗传差异,但总体供试材料遗传基础相对较狭窄。

The genetic diversity of 77 maize P1811 relatives were studied by simple sequence repeats（SSR） markers to know the genetic relationship of self-bred line P1811.The results showed that 222 alleles were detected from the 25 SSR primers giving stable amplified band patterns.The average number of alleles per SSR locus were 8.88 with a range of 4~16.The value of polymorphism information content（PIC） for each SSR locus varied from 0.615 7 to 0.938 1with an average of 0.87.The 77 maize P1811 relatives were divided into seven groups by UPGMA method based on SSR fingerprinting.The clustered results were similar to that based on geographical resource and germplasm genealogy.The 77 maize P1811 relatives had certain genetic differences,but the total hereditary basis was relatively narrow.