响应曲面法优化麒麟菜卡拉胶碱处理工艺

Optimization technology of alkali processing of Eucheuma carrageenan by response surface methodology

采用响应曲面法研究了碱液质量浓度、浸泡时间和浸泡温度对麒麟菜(Eucheuma)卡拉胶的凝胶强度和产率的影响。在单因素试验基础上采用Box-Benhnken中心组合试验,以碱液质量浓度、浸泡时间和浸泡温度为影响因素,以凝胶强度和产率为响应值建立二次回归方程,通过响应面分析得到优化组合。结果显示,碱处理优化工艺参数为碱液质量浓度250 g.L-1、浸泡时间3.5 d和浸泡温度25℃,在此条件下提取的麒麟菜卡拉胶凝胶强度为721 g.cm-2(质量浓度10 g.L-1),产率为35.17%。

We studied the influence of 3 factors including alkali concentration, soaking time and temperature on gel strength （GS） and yield of Eucheuma carrgeenan by response surface methodology （RSM）. On the basis of single-factor investigation, Box-Benhnken central composite experiments were carried out to build the quadratic regression models for GS and yield with the above 3 factors, and the alkali processing technology was optimized by response surface analysis. The optimizing process parameters are： alkali concentration 250 g.L-1 , soaking time 3.5 d, soaking temperature 25℃. Under the optimal processing condition, the GS and yield of carrgeenan were 721 g.cm-2 （under concentration of 10 g.L-1） and 35.17% , respectively.