锌胁迫下宽叶山蒿的耐性与富集特征

Effect of Zinc Stress on Growth and Zn Accumulation of Artemisia stolonifera

采用室内盆栽试验研究宽叶山蒿(Artemisia stolonifera)在Zn胁迫条件下的耐性和富集特征。结果表明,当w(Zn)≤500 mg.kg-1时,宽叶山蒿株高、根长和生物量均先随土壤中Zn浓度的升高而增加,之后则随Zn浓度的升高,Zn对宽叶山蒿的胁迫作用渐趋明显。与对照相比,当w(Zn)为1 500～3 500 mg.kg-1时,宽叶山蒿根部POD活性显著增大,当w(Zn)为3 500 mg.kg-1时,根部CAT活性显著增大;而叶片POD和CAT活性整体变化较不明显。宽叶山蒿根部和地上部w(Zn)最大值分别为999和3 214 mg.kg-1,对Zn的最大富集量分别为3.40和17.33 mg.盆-1。宽叶山蒿地上部对Zn的富集系数和迁移系数分别为0.26～0.82和0.65～1.05。可见,宽叶山蒿是一种对Zn具有较强耐性和富集潜力的植物,可用于Zn污染土壤的修复。

A pot experiment of Artemisia stolonifera was conducted to investigate its tolerance to and accumulation of Zn under Zn stress.Results show that its plant height,root length and biomass all increased with the increasing Zn concentration till Zn reached up to 500 mg·kg-1 in the soil and then declined with the rising Zn concentration in the soil,showing an obvious effect of Zn stress on the plant.Compared to control,the treatment of 1 500-3 500 mg·kg-1 Zn was much higher in POD（peroxidase） activity in roots and the treatment of 3 500 mg·kg-1 Zn was in CAT（catalase） activity in roots,however,no significant changes in POD and CAT activities in leaves were found.The highest Zn concentrations in root and shoot were found to be 999 and 3 214 mg·kg-1,respectively,and the largest amount of Zn accumulation was 3.40 mg·pot-1 in root and 17.33 mg·pot-1 in shoot.The Zn bioaccumulation coefficient was 0.26-0.82 and the Zn translocation coefficient 0.65-1.05,in shoot.Obviously,Artemisia stolonifera is a kind of plant high in tolerance to Zn and in Zn enrichment potential and can be used to remedy Zn polluted soils.