湖北省油茶种质资源的遗传基础研究

Analysis of Genetic Basis of Camellia oleifera from Hubei Province

用ISSR和SRAP 2种分子标记技术对湖北省5个地区的48个油茶资源进行了遗传多样性和亲缘关系分析。结果表明,无论是ISSR分析、SRAP分析,还是ISSR+SRAP的组合分析均显示油茶群体具有较高的遗传多样性。ISSR的6条引物共产生74条扩增带,多态性条带70条,多态性比例为94.59%;SRAP的11对引物组合共产生64条扩增带,其中有60条多态性带,多态性比例平均为93.75%;ISSR+SRAP的组合分析多态性比例为94.20%。2种分子标记及其组合所作的分类趋势基本相同,亲缘关系分析结果显示将5个地方居群分为2个类群,武汉新洲、黄冈红安、咸宁横沟桥和黄石阳新居群为一类,而恩施鹤峰居群单独为一类。

The diversity and genetic relationship among and within 5 populations of Camelliaoleifera from Hubei province were analyzed using ISSR and SRAP markers.High percentage ofpolymorphism observed by ISSR,SRAP or ISSR＋SRAP combination indicated a high level ofgenetic diversity existed among C.oleifera populations.Six ISSR primers yielded 74 legible bands of which 70 were polymorphic markers,and the ratio of polymorphism was 94.59%;A total of 64 SRAP bands（including 60 polymorphic bands） were detected with 11 SRAP primer combinations,and the average proportion of polymorphic loci was 93.75%;The percentage of polymorphic bands was 94.20% with ISSR＋SRAP combination.The two kind of DNA markers and their combination revealed similar genetic relationship for the dendrogram that the tested populations were divided into two groups：group Ⅰ was composed of 4 populations of WHXZ,HGHA,XNHGQ and HSYX,and group Ⅱ was consisted of one population of ESHF.