肝细胞癌组织中P16蛋白表达与丙型肝炎病毒感染的关系

Relationship of P16 protein expression with hepatitis Cvirusinfection in hepatocellular carcinom a

目的：研究肝细胞癌（Ｈｅｐａｔｏｃｅｌｌｕｌａｒｃａｒｃｉｎｏｍ ａ，ＨＣＣ）组织中Ｐ１６ 蛋白表达与丙型肝炎病毒（Ｈｅｐａｔｉｔｉｓ Ｃｖｉｒｕｓ，ＨＣＶ）感染的关系。方法：用免疫组化ＡＢＣ法检测４６例ＨＣＣ及其癌旁肝组织中Ｐ１６ 蛋白和ＨＣＶ抗原的表达。结果：Ｐ１６蛋白的阴性表达率在ＨＣＣ癌组织（５８．７％ ，２７／４６）明显高于其癌旁组织（３６．８％ ，１４／３８）（Ｐ＜ ０．０５），在Ｅｄｍ ｏｎｄｓｏｎ Ⅲ／Ⅳ级ＨＣＣ（７８．９％ ，１５／１９）明显高于Ⅰ／Ⅱ级ＨＣＣ（４４．４％ ，１２／２７）（Ｐ＜ ０．０５），在ＨＣＶ抗原阳性组（８０．０％ ，１６／２０）明显高于ＨＣＶ抗原阴性组（４２．３％ ，１１／２６）（Ｐ＜０．０５）。结论：Ｐ１６ 蛋白的表达与ＨＣＣ发生发展关系密切，ＨＣＶ致癌过程可能与细胞周期负性调节因子Ｐ１６基因功能受抑有关。

Objective: To study the relationship betwee P16 protein expression and hepatitis C virus (HCV) infection in hepatocellular carcinom a (HCC). Methods: P16 protein expression and HCV antigens w ere exam ined in the tissue sam ples of 46 cases of HCC and 38 sam ples ofperi-carcinoma tissues w ithim munohistochem ical and avidin-biotin complex m ethods. Results: The rate of negative P16 protein expression was significantly higher in the carcinomatous tissue (58. 7% , 27/46) than in the pericarcinom atoustissues (36.8% ,14/38) and in Edm ondson grade Ⅲand ⅣHCC(78.9% ,15/19) than in Edm ondson grade Ⅰand ⅡHCC(44.4% ,12/27) (P< 0.05). The rateofnegativeP16 protein expression w as significantly higherin HCV antigen positive group (80% ,16/20) than in HCV antigen negative group (42.3% ,11/26) (P< 0.05). Conclusion: P16 protein expression seem s to be related closely to the pathogenesis and developm ent ofHCCand HCV antigen may be involved in its pathogenesis through the inhibition on the function ofP16 gene, which exertsa negativeregulation ofcellproliferation ofHCC.