摘要
目的研究依达拉奉(Ed)对海人酸(KA)诱导癫痫大鼠海马组织硫氧还蛋白还原酶(TrxR)活性的影响。方法 SD大鼠随机分为癫痫对照组(saline组)、癫痫组(KA组)和药物对照组(KA+saline组)及药物组(Ed+KA组)。采用脑室注射KA制作大鼠癫痫模型,Ed于KA注射前40 min腹腔用药。KA注射6h取海马组织匀浆,应用DTNB还原法对海马TrxR活性进行测定,用Western blot法检测cleaved caspase-3的表达。结果 Saline组、KA组、KA+saline组、Ed+KA组TrxR活性分别为(U/mg protein):66±18、111±42、102±40、75±12;KA组和KA+saline组cleaved caspase-3的表达均较saline组增加(均P<0.05),而Ed+KA组较KA+saline组则降低(P<0.05)。结论 KA诱导癫痫大鼠海马组织TrxR活性增加,依达拉奉可能通过抑制TrxR的活性以增加对神经元的保护作用。
Objective To study the effect of edaravone on thioredoxin reductase（TrxR） activity of hippocampus in rats with seizure induced by kainic acid（KA）.Methods Adult male Sprague-Dawley rats weighing 200 to 300g were randomly divided into 4 groups： the control group（saline group）,the seizure group（KA group）,the vehicle treatment group（KA＋saline group） and the edaravone treatment group（Ed＋KA group）.Seizures were induced by intracerebroventricular injection of KA dissolved in sterile saline.Edaravone was intraperitoneally administrated to the rats 40 min before KA injection.TrxR activity was detected by dithio-bis-nitrobenzoic acid（DTNB） reduction assay.Western blot was used to analyze the expression of cleaved caspase-3.Results Thioredoxin reductase activity（U/mg protein）was 66±18,111±42,102±40 and 75±12.Compared with the saline group,the expression of cleaved caspase-3 increased significantly in the KA group and the KA＋saline group（P0.05）.Compared with the KA group and the KA＋saline group,the expression of cleaved caspase-3 decreased significantly in the Ed＋KA group（P0.05）.Conclusion TrxR activity increased in hippocampus of rats with seizure induced by KA.Edaravone may exert its neuroprotective effect by inhibiting the increasing of thioredoxin reductase activity.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2011年第5期740-742,805,共4页
Suzhou University Journal of Medical Science
基金
徐州市科技发展项目(XF10C058)
