毛葡萄叶水提物抑制血小板聚集的初步研究

Preliminary Study on Effects of Extracts of Vitis Quinquangularis Rehd Leaves on Inhibition of Platelet Aggregation

目的探讨毛葡萄叶水提物(Gx)对血小板聚集的影响。方法分离健康人的血小板,与不同生药浓度的Gx于37℃孵育10 min后,检测胶原(collagen)和凝血酶(thrombin)诱导的人血小板聚集的变化。小鼠腹腔注射Gx后,分离小鼠血小板,检测二磷酸腺苷(ADP)诱导的血小板聚集的变化。结果 Gx对2μg/ml collagen诱导的血小板聚集有显著抑制作用(P<0.05),且呈现Gx的剂量依赖性(P<0.05);只有0.01 mg/ml的Gx对0.1 U/mlthrombin诱导的血小板聚集有抑制作用,且不呈现Gx的剂量依赖性。同时,Gx可抑制ADP诱导的腹腔注射Gx的小鼠血小板聚集,且呈现ADP剂量依赖性。结论 Gx具有明显抗血小板聚集作用,为开发抗血栓药物提供了实验依据。

Objective To study the effect of extract of Vitis quinquangularis Rehd leaves（Gx） on platelet aggregation.Methods Isolated healthy human platelets were incubated with Gx at different concentration for 10 minutes at 37 ℃,and were stimulated with collagen and thrombin,then platelet aggregation was performed.After intraperitoneal injection of Gx,isolated mouse platelets were stimulated with ADP to examine the change of platelet aggregation.Results Human platelet aggregation incuced by 2 μg/ml collagen was dose-dependently inhibited by Gx（P0.05）;but only 0.01 g/ml Gx was able to inhibit human platelet aggregation induced by 0.1 U/ml thrombin,in a Gx dose-undependent way.And Gx was able to inhibit platelet aggregation induced by ADP in the intraperitoneally injected mice.Conclusion Gx can obviously inhibit platelet aggregation,and it can provide a experimental basis for development of antithrombotic drugs.