从何首乌提取物中制备二苯乙烯苷纯品的研究

Study on Preparation of 2,3,5,4"-Stilbene Glucosid from Polygoni Multiflori Radix

目的用层析柱分离、逆流色谱分离过程集成方法制备二苯乙烯苷纯品。方法从二苯乙烯苷含量小于4%的何首乌提取物中,通过层析柱分离制得20%左右纯度的样品,再通过逆流色谱分离制备二苯乙烯苷纯品。结果制得99%以上纯度的二苯乙烯苷样品。结论方法可靠,在避光的情况下纯品稳定。

Objective To prepare pure 2,3,5,4＂-stilbene glucosid from Polygoni Multiflori Radix by the method of Column Chromatography and high-speed counter-current chromatography（HSCCC）.Methods 20% 2,3,5,4＂-stilbene glucosid was prepared by Column Chromatography,and the pure 2,3,5,4＂-stilbene glucosid was prepared by HSCCC.Results Over 99% 2,3,5,4＂-stilbene glucosid was obtained.Conclusion The method had good reproducibility and the purification of 2,3,5,4＂-stilbene glucosid was stability from sunlight.