杀鞘翅目苏云金芽孢杆菌新菌株及其杀虫剂的研究

NEW COLEOPTERANCIDAI STRAINS OF BACILIUS THURINGIENSIS AND PRODUCTION OF COLEOPTERANCIDE

从中国土壤中分离出２ 株杀鞘翅目昆虫的苏云金芽孢杆菌（ Ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ）ＹＭ０３ 及ＳＨＱ１１１０ 。ＹＭ０３ 的血清型为Ｈ８ａ８ｂ ，ＳＨＱ１１１０ 的Ｈ 血清型未知。二菌株皆产近菱形的薄扁伴孢晶体，分别含６８ ～７０ｋＤ 和６５ｋＤ 的晶体蛋白质。毒力生物测定证明对柳蓝叶甲（ Ｐｌａｇｉｏｄｅｒａ ｖｅｒｓｉｃｏｌｏｒａ） 及马铃薯甲虫（ Ｌｅｐｔｉｎｏｔａｒｓａ ｄｅｃｅｍｌｉｎｅａｔａ） 有高毒效。发酵性能良好。ＹＭ０３ 粉剂田间防治马铃薯甲虫有高效。稀释４００ 倍喷雾，防治效果达９４６ ％ 。

Two coleopterancidal strains of Bacillus thuringiensis , YM 03 and SHQ11 10, were isolated from soil in China. H Serotype of YM 03 was H8a8b,and H Serotype of SHQ11 10 has not been identified. Both of them produced flat bipyramid like crystals which consisted of 68～70kD and 65kD protien respectively. Bioassay indicated that these two Bt strains were very active on the larva of Leptinotarsa decemlineata and Plagioder aversicolora . They grew prosperously in fermentation. Powder of YM 03 product was high effect against colorado potato beetle in the field experiment.