固定化青霉素V酰化酶的制备及性质

PREPARATION OF IMMOBILIZED PENICILIN V ACYLASE FROM FUSARIUM OXYSPORUM AND ITS PROPERTIES

尖镰孢（ Ｆｕｓａｒｉｕｍ ｏｘｙｓｐｏｒｕ ｍ）ＦＰ９４１ 青霉素Ｖ 酰化酶经γ氧化铝吸附洗脱、硫酸铵沉淀和脱盐处理后，固定在环氧丙烯聚合物载体上，湿固定化酶表现活力为２１７ ＩＵ／ｇ ，固定化产率为５３ ％ 。固定化酶作用最适温度为５５ ℃，最适ｐＨ 为８０ ；在ｐＨ５０ ～１１０ 及５０ ℃以下稳定；３７ ℃使用２５ 次后，酶活力保留９０ ％ 。

Extracellular pencillin V acylase from Fusarium oxysporum FP941 was partially purified by means of adsorption on, and elution from γ alumina and (HN 4) 2SO 4 fractional precipitation. The enzyme was immobilized on acrylic polymer support linkaged with covalent bond. The activity of wet immobilized enzyme was 217 IU/g, The activity yield of Immobilized enzyme was 53%. Optimum pH and temperature for immobilized enzyme action wer 8 0 and 55℃, respectively. The enzyme was stable below 50℃ and pH?4～11. The activity of the immobilized enzyme remained 90% after it was reused 25 times.