ω-3多不饱和脂肪酸对失血性休克大鼠肠道细菌移位及存活时间的影响

Effect of ω-3 polyunsaturated fatty acid on enteral bacterial translocation and survival time of rats subjected to hemorrhagic shock

目的探讨ω-3多不饱和脂肪酸(PUFA)对失血性休克大鼠肠道细菌移位及存活时间的影响。方法 Wist-ar大鼠60只,随机分成假手术组(SHS)、失血性休克组(HS)及失血性休克+PUFA干预组(HS-F),每组各20只;观察各组大鼠存活时间、存活率及平均动脉压(MAP)动态变化,取肝、脾、肾及肠系膜淋巴结做细菌培养。结果 SHS、HS、HS-F组大鼠的存活时间分别为>180min、(113.0±25.9)min、(138.4±37.1)min,HS组与HS-F组大鼠的存活时间比较,差异有统计学意义(P<0.01);HS组及HS-F组较SHS组肝、脾、肾及肠系膜淋巴结细菌移位率明显升高(P<0.01);HS-F组较HS组肝、脾、肾及肠系膜淋巴结细菌移位率明显降低(P<0.01)。结论脂肪乳对HS大鼠肠黏膜屏障具有明显的保护作用,可有效降低HS后肠道细菌移位,并显著延长HS大鼠的存活时间。

OBJECTIVE To investigate the effects of ω-3 polyunsaturated fatty acid（PUFA） on enteral bacterial translocation and survival time in rats subjected to hemorrhagic shock. METHODS Totally 60 male wistar rats were randomly divided into 3 groups（n=20）： Sham hemorrhagic shock group（SHS）;hemorrhagic shock group（HS） and hemorrhagic shock with fat emulsion group（HS-F）.The bacterial translocation from liver,spleen,kidney and intestinal lumen,the survival time,survival rate and mean artery pressure（MAP） were examined. RESULTS The bacterial translocation rate in liver,spleen,kidney and intestinal lumen were significantly higher in HS-F group and HS group than those of SHS group（P0.01）.Compared with HS group,the bacterial translocation rate was significantly lower and the survival time of HS-F was longer in HS-F group（P0.01）. CONCLUSION PUFA is beneficial to the protection of the intestinal mucosa,significantly decreases intestinal bacterial translocation and prolonged survival time in rats subjected to HS.