摘要
为培育去除选择标记基因的耐旱转基因植物,同时利用Cre/Lox和FLP/frt系统,构建一个能够高效删除标记基因的Bhlea2基因植物表达载体。拟南芥rd29A启动子是在低温、干旱、高盐胁迫下的快速应答启动子,玉米ubiquitin启动子可有效驱动外源基因的转录,拟南芥pAB5启动子是花粉及胚胎等发育早期特异表达的启动子,利用上述启动子构建了表达Bhlea2基因并能够删除标记基因的植物表达载体。该表达载体包括重组酶表达元件pAB5-FLP、Bhlea2抗旱基因表达元件rd29A-Bhlea2和bar标记基因表达元件ubiquitin-bar。
For breeding the marker-free transgenic plants with high tolerance to drought,a Bhlea2 gene vector with efficient deleting marker gene system was constructed using the system of Cre/Lox and FLP/frt.Rd29A promoter is a rapid responsive promoter from Arabidopsis thaliana under the condition of low temperature,drought and high salt stress.The promoter of the maize ubiquitin gene can efficiently regulate the transcription of a foreign gene in transgenic plants.pAB5 promoter of Arabidopsis thaliana expresses especially at the early development stage of pollen and embryo.Using the promoter of above,we constructed a Bhlea2 gene vector with efficient deleting marker gene system,the vector contained the elements of pAB5-FLP,rd29A-Bhlea2 and ubiquitin-bar.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第11期79-82,共4页
Biotechnology Bulletin
基金
创新能力建设专项(KJCX201102003)
转基因重大专项(2009ZX08009-060B)
国家高技术研究发展计划"863"计划(2009AA10Z107)
北京主要作物高效育种关键技术的合作研究(2010DFB33740)
辽宁省教育厅高等学校科学技术研究项目(2008663)
关键词
位点特异性重组系统
标记基因删除
转基因植物
Site-specific recombination system Deletion marker gene Transgenic plants