高效删除标记基因的Bhlea2植物表达载体的构建

Construction of a Bhlea2 Gene Vector with Efficient Deleting Marker Gene System in Transgenic Plants

为培育去除选择标记基因的耐旱转基因植物,同时利用Cre/Lox和FLP/frt系统,构建一个能够高效删除标记基因的Bhlea2基因植物表达载体。拟南芥rd29A启动子是在低温、干旱、高盐胁迫下的快速应答启动子,玉米ubiquitin启动子可有效驱动外源基因的转录,拟南芥pAB5启动子是花粉及胚胎等发育早期特异表达的启动子,利用上述启动子构建了表达Bhlea2基因并能够删除标记基因的植物表达载体。该表达载体包括重组酶表达元件pAB5-FLP、Bhlea2抗旱基因表达元件rd29A-Bhlea2和bar标记基因表达元件ubiquitin-bar。

For breeding the marker-free transgenic plants with high tolerance to drought,a Bhlea2 gene vector with efficient deleting marker gene system was constructed using the system of Cre/Lox and FLP/frt.Rd29A promoter is a rapid responsive promoter from Arabidopsis thaliana under the condition of low temperature,drought and high salt stress.The promoter of the maize ubiquitin gene can efficiently regulate the transcription of a foreign gene in transgenic plants.pAB5 promoter of Arabidopsis thaliana expresses especially at the early development stage of pollen and embryo.Using the promoter of above,we constructed a Bhlea2 gene vector with efficient deleting marker gene system,the vector contained the elements of pAB5-FLP,rd29A-Bhlea2 and ubiquitin-bar.