摘要
目的:观察16天的预热适应对大鼠运动性骨骼肌损伤的影响;方法:成年大鼠80只,随机分为非预热适应组和预热适应组,各组又分为安静对照组、运动后即刻、运动后24h、运动后48h组和运动后6d组。预热适应模型:热刺激30～35min/天,共16天。末次热适应结束后,室温恢复24h,除安静对照组外,其余大鼠均进行一次离心运动至动物宰杀。取腓肠肌,进行光镜观察和HSP70表达测试;结果:1)预热适应运动大鼠骨骼肌损伤较单纯运动大鼠相应的时间点明显减轻;2)预热适应运动大鼠运动后即刻和运动后48hHSP70表达分别高于单纯运动后相对应时间点的值(P<0.05);结论:预热适应对大鼠运动性骨骼肌损伤有明显保护作用,其机理可能与长期(16天)的预热适应能上调HSP70蛋白表达有关。
Objective：To investigate the effects of heat pretreat adaptability of 16 days on skeletal muscle injury induced by an eccentric exercise. Methods： 80 male Sprague-Dawley （SD） rats were randomly divided into non-preheat adapted groups and preheat adapted groups, each of which was further divided into quiet group, immediately after exercise group, 24 hours after exercise group,48 hours after exercise group, and 6 days after exercise group. Rats in heat groups were subjected to whole-body hyperthermia for 16 days （ 30- 35 min / d of heat stimulation） and were allowed to recover from the last whole-body hyperthermia for 24 hours. All the exercise groups performed an exhaustive exercise on a treadmill. The rats were killed on 0,1, 2,6 days after exercise and isolated gastrocnemius, which were sampled to be observed by light microscopy and measured HSP70 expression. Results： 1） The degrees of skeletal muscle injury in pre-heat adapted-exercise groups were markedly lower than that of simple exercise groups at corresponding time points. 2） HSP70 expression of rats immediately and 48 hours after exercise in preheat adapted-exercise groups increased significantly comparing with the simple exercise groups at corresponding time points respectively （P〈0.05） . Conclusion：Heat pretreat adaptability can protect against eccentric exercise-induced muscle injury, and the mechanism may be related to heat pretreat adaptability of 16 days which can up-regulate the expression of HSP70.
出处
《体育科学》
CSSCI
北大核心
2011年第12期73-78,共6页
China Sport Science
